Coding

Part:BBa_K1074000:Experience

Designed by: Changlong Zhao   Group: iGEM13_USTC_CHINA   (2013-09-09)

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Applications of BBa_K1074000

User Reviews

UNIQ0589db4bbbd4780f-partinfo-00000000-QINU UNIQ0589db4bbbd4780f-partinfo-00000001-QINU

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USTC_China iGEM13

In our project,we fused TD1 with a varity of antigens(HBsAg(Hepatitis B virus)BBa_K1074002,Ag85b(Mycobacterium tuberculosis)BBa_K1074003,PAD4(Bacillus Anthracis)BBa_K1074004 ),adjuvant LTB (BBa_K1074005)and GFP(BBa_E0040), induced expression in BL21 and Bacillus Subtilis WB800N with IPTG and tested positive by SDS-PAGE and ELISA. Then we validated the efficient transdermal function of TD1 fused antigen HBsAg by ELISA. Students in Wen lab also validated the efficient transdermal function of N-terminal TD1 modified GFP(not BBa_E0040)

figure 1 Our Transdermal test device
figure 2 ELISA test transdermal function of N-terminal TD1 modified antigen HBsAg


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